In this experiment I aim to find out the relationship between the leakage of red pigment from a beetroot cell and the surface area. If the pH levels were to be affected, the enzymes would not function efficiently thus allowing chemical reactions to take place. Another limitation was cutting the beetroot into pieces. A way to avoid the variation could be to use a device which has cutting utensils at a set distance which would produce pieces that were of equal length. Record the results for each reading in a table. To do this successfully I will need to alter the surface area of the beetroot cells accurately and then measure if any and how much dye is let out.
Wearing rubber gloves may also help this purpose as they give an extra layer. Control Variables: In order to make the test fair, only one person must time the amount of time the beetroot has been inside the concentrated detergent and the. Finally, the spectrophotometer had not been turned on when entering the lab so it might add to errors displayed in this trial. Equipment:- Beaker Thermometer Heat proof mat Tripod Bunsen Burner Cork Borer 10 Beetroot cylinders Forceps 10 test tubes Colorimeter Measuring cylinder Also to improve the reliability of the test I will repeat the results. For example, if there is a positive correlation between the results, I will be able to conclude that with a positive increase in temperature, there will be greater light absorption. What are the dependent variables? As the actual size was simply estimated at around 3mm the sample sizes between groups could have been much different.
Experiment One Temperature C Transmission of light absorption % Room temp88. The Reasoning behind the experiment is to see what effects Temperature has on the permeability of Phospholipid bi-layer of Beetroot cells and the amount of pigment released from the vacuole. Beet membranes that have a similar size and shape could have given better result on the distinctive solutions. However I am unsure if this will be an applicable method as above 40C the membrane becomes damaged. The visible spectrum of light is the portion of the electromagnetic spectrum which is visible to the human eye. This will make sure that the same period of time is given for dye leakage to occur. If the membrane is altered in any way, this may have an effect on its permeability and therefore the properties of the cell of which it is a part.
As surface area increases the rate of reaction which in turn would cause an increase in the rate of diffusion causing more dye to be given out. Independent Variable The independent variable in this investigation was the solutions in the petri dishes. There are many things that can affect what is transported in and out of the cell: temperature, pH, other chemicals solvents Middle 15. I predict that the rate of diffusion and ethanol concentration will be directly proportional. At temperatures above approximately 50ÃÂ°C proteins begin to get destroyed; this would therefore increase the permeability of both the cell wall and the vacuole wall.
Transmembrane proteins have hydrophilic and hydrophobic regions which cause the proteins to align in a specific way in the membrane. By using a machine to cut the perfect size of beet it could be guaranteed that each group would be working with equivalent beet product. I will do this by measuring the percentage light transmission using a spectrophotometer. This would be able to tell me whether or not my prediction is correct, and assure me of the reliability of my results. This would be done by changing the amount of time of which the beetroot sample is heated, and measuring its % transmittance of light. Hypothesis: If more temperature is added to the cellular membrane, then the color of each solution will be more intense.
Overall, my set of data collected is relatively reliable, as the experiment was conducted altogether 4 times in order to get more results for comparison and higher degree of accuracy. In chloroplasts and mitochondrion, they keep pigments and carrier molecules in fixed positions. When adding solutions to the beet cubes, we learned that the high-percentage solutions caused the beet to bleed more betacyanin than the low-percentage solutions did. After waiting twenty minutes for the solutions to settle, we found the data placed in Table 9. The closer the number is to 0, the less that is absorbed. Control The main variable in this experiment will be temperature as I am trying to find out how this specifically affects the cell membrane.
The pH's, which cause this affect, would be those that are acid or alkaline. Rinse away any pigmented water in the morning and replace with fresh water. Externally the cytoplasm is surrounded by the plasma membrane hence the foundation of the experiment depends… 5088 Words 21 Pages measuring the effects of temperature on the membrane permeability of beetroot. This would have had a slight effect on my results because the rate of diffusion of betalain particles across the plasma membrane is increased, as the surface area of the beetroot increases. The Beetroot discs will become flaccid and decrease in mass if the water potential of the surrounding solution is lower than the water potential inside the beetroot cell. I only looked at one type of plant cell, so I can not be sure that every plant cell and indeed eykaryote- which have different plasma membranes, that may be adapted to cope with heat better or worse than willl have the same results. A pH of about 3 - 4 stabilises the pigments and protects against oxidation.
When we finished we returned the beet to test tube three and added 10 mL of distilled water. Impermeability of Seed Coat 5. Without a fair test you will not receive accurate results, in turn, making the conclusions drawn from this investigation inaccurate. Using forceps, 5 beetroot cylinders were placed in every petri dish. I will do this by placing each beetroot piece onto the white tile and cutting it into small pieces measuring 20mm each. Evaluating Procedures: The method of this experiment was well written.
I can hopefully then look at my results and then find a relationship between the two factors and be able to explain exactly why any changes took place. The cubes of beet tissue were placed in a beaker and rinsed with tap water for two imiutes to wash the betacyanin from the injured cells on the surface. This is because the surface area affects the rate of diffusion, thus affecting the results. Thus from the data of %transmittance readings, it can be concluded that the longer the beetroot sample is heated, the more permeable it is. Determining the Degradation of a Root Cell Membrane for the Beta Vulgaris through the Absorbance while placed in Various Temperatures Introduction S.
Place all the discs into a beaker and wash with water until no more pigment is washed from the slices. This is so that can obtain a more accurate reading of light transmission. Prediction: The Prediction is that as the temperature rises as does the permeability of the cell membrane, as the permeability and the temperature increase so does the amount of pigment being released. The detergent got its most noteworthy absorbance readings of 5 in a concentration of 1%. Six petridishes are taken and the solutions are added in the following proportions: Equal slices of beet root of uniform thickness are cut with the help of a cork borer and these are thoroughly washed in distilled water to remove the adhering anthocyanin pigment from the wounded cells. Experiments on Differential Permeability of a True Solution and a Colloidal Solution 4.